Pyrosequencing enumerates and contrasts soil microbial diversity

Analysis of Microbial Diversity by Amplicon Pyrosequencing

Microbial diversity and soil functions

Soil is a complex and dynamic biological system, and still in 2003 it is difficult to determine the composition of microbial communities in soil. We are also limited in the determination of microbially mediated reactions because present assays for determining the overall rate of entire metabolic processes (such as respiration) or specific enzyme activities (such as urease, protease and phosphom...

Plant diversity increases soil microbial activity and soil carbon storage.

Plant diversity strongly influences ecosystem functions and services, such as soil carbon storage. However, the mechanisms underlying the positive plant diversity effects on soil carbon storage are poorly understood. We explored this relationship using long-term data from a grassland biodiversity experiment (The Jena Experiment) and radiocarbon ((14)C) modelling. Here we show that higher plant ...

Metagenomic pyrosequencing and microbial identification.

BACKGROUND The Human Microbiome Project has ushered in a new era for human metagenomics and high-throughput next-generation sequencing strategies. CONTENT This review describes evolving strategies in metagenomics, with a special emphasis on the core technology of DNA pyrosequencing. The challenges of microbial identification in the context of microbial populations are discussed. The developme...

Metagenomic approach to soil microbial diversity and functions

The improved methods to analyze DNA or RNA from soil enable us to study microbial diversity and functions in soil which have never been revealed by conventional culture methods. Using one of such metagenomic approaches, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), we succeeded in monitoring the change of microbial diversity in the soil treated with a chemicals o...